Monoclonal immunoglobulin A antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro
نویسندگان
چکیده
منابع مشابه
Immunological control mechanism against cholera toxin: interference with toxin binding to intestinal receptors.
The immunological control mechanism against cholera toxin (CT) in the small intestine of rats was studied in vivo. CT binding to intestinal receptors was determined by injected radiolabeled CT into the loops of rat small intestine and subsequently separating purified microvillus membranes from mucosal scrapings of those loops. substantial radioactivity (10(5) cpm/mg of microvillus protein) was ...
متن کاملProduction and characterization of monoclonal antibodies to cholera toxin.
Monoclonal antibodies against cholera toxin were produced to obtain highly specific antisera to cholera toxin. Fifteen hybridoma cell lines producing monoclonal antibodies specific for the determinants of cholera toxin were derived from the fusion of mouse myeloma cells and spleen cells from mice immunized with cholera toxin. The cell lines were stabilized, examined for specific antibody produc...
متن کاملBoth cholera toxin-induced adenylate cyclase activation and cholera toxin biological activity are inhibited by antibodies against related synthetic peptides.
The immune response against six synthetic peptides corresponding to various segments of the B subunit of cholera toxin was evaluated. Conjugates in which the peptides were covalently linked to tetanus toxoid served for immunization of rabbits. As previously reported, four of these conjugates elicited antibodies cross-reactive with intact cholera toxin. We report here that antisera against two o...
متن کاملCholera toxin notches epithelial junctions.
Cholera toxin (CT) is the factor responsible for watery diarrhea associated with Vibrio cholerae infection. In this issue, Guichard et al. (2013) report that CT compromises intestinal epithelium barrier function via cyclic AMP (cAMP)-induced disruption of Rab11- and exocyst-dependent delivery of endocytic recycling cargo to cell-cell junctions.
متن کاملSpecific binding of cholera toxin to isolated intestinal microvillous membranes.
A sucrose density gradient assay was used to demonstrate the specificity and saturation of the binding of [(125)I]cholera toxin to isolated intestinal microvillous membranes from rat small intestine. When the toxin is first complexed to antitoxin and then exposed to intestinal membranes, the binding of cholera toxin is inhibited. To emphasize the physiologic importance of these observations, si...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1993
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.61.12.5271-5278.1993